Safranine O , 25gm

Safranine O is a triphenylmethane dye that is chemically known as 3,7-Diamino-2,8-dimethyl-phenylphenazinium chloride. It is a crystalline solid with a characteristic green metallic lustre. It is readily soluble in water and dye red or violet. They are strong bases and form stable monacid salts. Their alcoholic solution shows a yellow-red fluorescence.

Safranine O is used in a variety of staining protocols, including:

• Gram stain: Safranine O is used as a counterstain in the Gram stain, which is a staining procedure used to differentiate between Gram-positive and Gram-negative bacteria.
• Endospore stain: Safranine O is used as a counterstain in the endospore stain, which is a staining procedure used to visualize bacterial endospores.
• Fungal stain: Safranine O is used as a counterstain in the fungal stain, which is a staining procedure used to visualize fungi.
• Cell culture stain: Safranine O is used as a counterstain in cell culture stains, which are staining procedures used to visualize cells in culture.

Safranine O is a safe and effective dye when used properly. However, it can be irritating to the skin and eyes. It is important to wear gloves and eye protection when handling safranine O.

Here are some safety precautions to take when using safranine O:

• Wear gloves and eye protection when handling safranine O.
• Avoid breathing in dust or mist from safranine O.
• If safranine O gets on your skin, wash it off immediately with soap and water.
• If safranine O gets in your eyes, flush them with water for at least 15 minutes and seek medical attention immediately.
• Keep safranine O out of reach of children and pets.