Leishman stain , 25gm

The Leishman stain is named after its inventor, Dr. W. Leishman, who developed it in 1903. The stain is still used today to diagnose leishmaniasis, a tropical disease caused by Leishmania parasites.

It is a simple and inexpensive staining technique that can be performed in a laboratory. The technique is as follows:

1. The tissue sample is fixed in methanol.
2. The tissue sample is stained with Leishman’s solution, which is a mixture of methylene blue and eosin.
3. The tissue sample is washed with water.
4. The tissue sample is examined under a microscope.

The Leishman stain is a sensitive staining technique that can detect even small numbers of Leishmania parasites. However, it is not specific for Leishmania parasites, and other types of cells and structures can also be stained red. Therefore, the Leishman stain should be used in conjunction with other diagnostic tests to confirm the presence of leishmaniasis.

Here are some of the advantages of the Leishman stain:

• It is a simple and inexpensive staining technique.
• It is sensitive and can detect even small numbers of Leishmania parasites.
• It is widely available and can be performed in most laboratories.

Here are some of the disadvantages of the Leishman stain:

• It is not specific for Leishmania parasites.
• It can be time-consuming to perform.
• It can be difficult to interpret the results.