Acridine Orange: A Versatile Fluorescent Stain for Nucleic Acids
Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye commonly used in various biological applications. It is known for its unique property of emitting different colors depending on its binding to DNA or RNA. This makes it a valuable tool for researchers studying cell cycle, apoptosis, and other cellular processes.
Product Description:
- CAS Number: 494-38-2
- Molecular Formula: C₁₇H₁₉N₃
- Appearance: Orange powder
- Solubility: Water
Key Features:
- Stains double-stranded DNA (dsDNA) green and single-stranded DNA (ssDNA) or RNA red
- Cell-permeable, allowing for easy staining of live cells
- High sensitivity and specificity for nucleic acids
- Compatible with various fluorescence microscopy techniques
Applications:
- Cell cycle analysis
- Apoptosis detection
- RNA visualization
- Microorganism detection
- Lysosomal staining
- Autophagy detection
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