Folin & ciocalteu’s phenol reagent , 500ml

EGP 950.00

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  • Folin–Ciocalteu reagent (FCR), also known as Folin’s phenol reagent or Folin–Denis reagent, is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants, also called the gallic acid equivalence method (GAE).

Category:

Product Name: Folin & Ciocalteu’s Phenol Reagent

Chemical Composition: Folin & Ciocalteu’s Phenol Reagent is a mixture of phosphomolybdic acid and phosphotungstic acid.


Description:

Folin & Ciocalteu’s Phenol Reagent, also known as Folin-Ciocalteu reagent, is a widely used chemical reagent in analytical chemistry for the determination of total phenolic content in various substances. It is named after the chemists Otto Folin and Vintilă Ciocâlteu, who developed the reagent. This reagent works by reacting with phenolic groups under alkaline conditions to form a blue-colored complex, which can be quantified spectrophotometrically. It is highly sensitive and essential for assessing the antioxidant capacity and phenolic composition of samples.


Applications:

  1. Analytical Chemistry:
    • Used in the Folin-Ciocalteu assay to quantify total phenolic content in extracts, beverages (such as wine and tea), and food samples.
    • Provides valuable insights into the antioxidant capacity and nutritional quality of foods and beverages.
  2. Research and Development:
    • Essential in pharmaceutical and nutraceutical research for evaluating the antioxidant properties of compounds.
    • Used in environmental studies to assess phenolic pollutants and natural phenolic compounds in water samples.

Specifications:

  • Appearance: Dark blue solution
  • Composition: A mixture of phosphomolybdic acid and phosphotungstic acid in aqueous solution.
  • Solubility: Soluble in water
  • Storage Conditions: Store in a cool, dry place, away from light and heat sources. Keep containers tightly closed to prevent contamination.

Advantages:

  • High Sensitivity: Detects a wide range of phenolic compounds with high sensitivity.
  • Versatility: Suitable for use in various sample matrices, including aqueous extracts and organic solvents.
  • Quantitative Analysis: Provides quantitative results based on spectrophotometric measurements.

Safety Information:

  • Hazards: Avoid direct contact with skin and eyes. May cause irritation.
  • Handling: Use in a well-ventilated area. Wear appropriate personal protective equipment (PPE), including gloves and safety goggles.
  • Disposal: Dispose of according to local regulations for chemical waste.

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