Ninhydrin AR (analytical reagent grade) is a white or pale yellow crystalline powder with a molecular formula of C9H4O3.H2O and a molecular weight of 178.14. It is a pH indicator that turns blue in the presence of primary amines. Ninhydrin is used in a variety of applications, including:
- For the detection of amino acids and proteins in thin-layer chromatography (TLC) and paper chromatography
- For the identification of fingerprints
- For the determination of the concentration of amino acids in biological fluids
- As a reagent in the ninhydrin test for carbohydrates
- As a reagent in the ninhydrin test for steroids
Ninhydrin AR is a safe and stable compound when handled properly. However, it is important to note that it is a strong oxidizing agent and can cause skin irritation if it comes into contact with skin. It is also important to store ninhydrin AR in a cool, dry place away from heat and light.
Here are some additional details about ninhydrin AR:
- It has a melting point of 235-237°C.
- It is soluble in water, ethanol, and methanol.
- It is insoluble in hexane and ether.
- It has a characteristic odor of almonds.
- It is a strong reducing agent.
- It is toxic if ingested.
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